| 1. | Plasmid pucis was used to construct viral genome library . ten fragments were obtained and some of them were partially sequenced and analysed using genbank data
利用puc18质粒构建了织线藻病毒部分基因组的dna文库，获得了10个基因组片段，作了部分克隆片段的序列测序和分析。 |
| 2. | The centre of excellence , located at the hepatitis research laboratory in the prince of wales hospital , can offer viral genome sequencing for patients who receive anti - viral therapy
中心于威尔斯亲王医院的肝炎研究实验室，可以为接受抗病毒治疗的乙肝病人进行病毒基因组排序。 |
| 3. | There are many mutations leading to drug resistance exist in the viral genome which varies in different individuals especially in those receiving different anti - viral drugs
不同乙肝病人在接受不同程度的抗病毒药物治疗后，病毒会出现各式各样的变异，导致病毒基因组产生抗药性。 |
| 4. | It refers to the release of the viral genome from its protective capsid to enable the nucleic acid to be transported within the cell and transcribed to form new progeny virions
它指的是将病毒基因组从它的保护性衣壳中释放出来，使核酸能在细胞内转运并能转录以形成新的子代病毒。 |
| 5. | Chapter two is a report on genetic organization of the hindlll - l fragment of hasnpv . the fragment that is 7501 bp long and located at map units ( mu ) 12 . 3 - 17 . 9 of the viral genome was sequenced
对棉铃虫单核衣壳核多角体病毒( hasnpv )的研究历史和近期对hasnpv的基因组学研究以及本论文的研究内容作了简要介绍。 |
| 6. | ( 2 ) assay of rdrp activity and immunoreactivity of the recombinant ns5 first we respectively cloned the 5 " and 3 " terminal region ( tr ) sequence of the viral genome by rt - pcr and jointed the two segment by ligation
( 2 )重组nss蛋白rdrp活性和抗原性的分析:通过rt一pcr分别获得den基因组5 ’末端区( tr )和3 ‘一r的cdna片段，将两片段连接，形成两端与原病毒基因组trs序列相同的亚基因组dna 。 |
| 7. | Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis . the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation , 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain . referred to the published sequence two primers were designed and synthesized
给4周龄spf雏鸡人工接种鸡传染性法氏囊病病毒hb - bp毒株， 48小时后采取法氏囊，用双licl分级沉淀法提纯全长基因组dsrna ，设计一对引物，通过rt - pcr方法进行了体外扩增，获得hb株a节段基因全长cdna 。 |
| 8. | But such research on virus is still falling behind relatively . in order to probe the malignancy of virus and its toxicology to cell and tissue , to analyze the function of main structural genes of viral genome in pathogencity and immune , to manufacture drugs , which prevent the virus with bacteria and to effective vaccine , genome must be analyzed
而对病毒这方面的研究相对滞后，为了探讨病毒对细胞、组织的毒性作用及其毒理，分析病毒基因组中主要结构基因在致病和免疫中的功能，研制阻断病毒与细胞融合的药物及其有效的疫苗，为其防制提供全新的思路，就必须以基因组的分析为基础。 |
| 9. | In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera , the ecori - n fragment located at 54 . 8 - 59 . 3 kbp of the viral genome was sequenced . the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda
对棉铃虫单核衣壳核多角体病毒( helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv )基因组中ecori ? n片段进行序列分析，获得了完整的解螺旋酶基因( hel ) ，其开放阅读框大小为3762bp ，编码一个分子量为146kda的蛋白质。 |
| 10. | 2 . an up - dated method was employed to purify tumv in this research . using the protease k method , we acquired the viral genome - rna . a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates
应用改进的芜菁花叶病毒的提取方法从病叶中提取病毒粒体，应用蛋白酶k法从病毒粒体中提取病毒rna基因组，根据已报道的tumv的cp基因序列，设计并合成了一对特异引物，利用rt - pcr法克隆了6个分离物的外壳蛋白基因，与克隆载体puc19连接后通过热激法转化大肠杆菌dh5 。 |
